Data are expressed as averages plus standard deviations (SD) from three independent experiments (P <0.05) a statistically significant difference of the different experimental points. Peptides were diluted in ultrapure water and positive control (bacterium and Mueller Hinton broth). The data are expressed as averages plus standard deviations (SD) from two independent experiments (P <0.01).Įvaluation of the anti-amastigote activity in Leishmania amazonensis and cytotoxicity with mouse peritoneal macrophages Swiss.Īntibacterial assays of the peptides RP1 and Fc-RP1 in S. amazonensis intracellular amastigotes not treated with the compounds. The infection index was calculated after 24 h treatment at the IC 50 of each compound. In vitro effect of a) RP1 and ferrocene carboxylic acid and b) Fc-RP1 and Amphotericin B against L. The peptides were evaluated in concentration of 60 μmol L -1 dissolved in PBS. (c) Peptides in POPC: POPS vesicles (9:1 / m:m) also at the concentrations of 300 and 150 μmol L -1 of lipids. (b) Peptides in POPC unilamellar vesicles at the concentrations of 300 and 150 μmol L -1 of lipids.

(a) RP1 and Fc-RP1 in PBS solution and LPC at concentrations of 1 and 5 mmol L -1.

Anova post hoc in graphpad prism 8